Triazole Fungicides Sensitivity of Sclerotinia homoeocarpa in Korean Golf Courses

Chemical management of dollar spot in turf may lead to the development of Sclerotinia homoeocarpa populations with reduced fungicide sensitivity. The objective of this study was to investigate resistance of S. homoeocarpa isolates to triazole fungicides and to test cross-resistance among three triazole fungicides. A total of 66 isolates of S. homoeocarpa were collected from 15 golf courses across Korea, and tested via in vitro sensitivity assay against hexaconazole, propiconazole and tebuconazole. EC50 values of the isolates to these fungicides were distributed in the range of 0.001-1.1 a. i. μg ml-1. Based on the EC50 values, twelve representative strains were selected as sensitive isolates including control and insensitive isolates with respect to each fungicide. At a concentration of 0.1 a. i. μg ml-1 for all fungicides, the selected strains were distinguished as sensitive or resistant isolates with the mycelial growth inhibition rate of 50% as the criterion. The EC50 values of resistant strains exposed to hexaconazole, propiconazole and tebuconazole were 20-50 times, 50-70 times, and 77 times greater, respectively, than that of the control strains. Two isolates of S. homoeocarpa S0-41 and Sh14-2-1 showed sensitivity toward all the fungicides used, while two other isolates Sh7-5-1 and Sh2-1-1 showed resistance to all fungicides. Each isolate showed similar resistance to the three types of triazole fungicides, whereby cross-resistance of isolates was confirmed in the present study; all three triazole fungicide combinations displayed significant correlation coefficients equivalent to or greater than 0.8.